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The pKa, of imidazole rings varies according to the microenvironment that protein folds in, and this huge pKa fluctuation enables the proteins histidine binding with metals (Miyagi and Nakazawa, 2024)
Mitochondrial membrane potential ( m ) determination and acidic organelle tracking Cells were seeded in a 24-well plate and incubated with 100 nM TMRM or LysoTracker Red DND-99 or 33 M of acridine orange for 30 min at 37 C in phenol-free medium (and addition of Hoechst)
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