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Sometimes this issue is fixed simply by optimizing buffers, for instance by increasing salt or detergent concentration, so that the interacting protein no longer binds to the GST only control, but still binds to your GST-fusion protein of interest

GH peak/insulin-like growth factor-1 (IGF-1) were also significantly reduced in severe ME/CFS patients compared to controls and ME/CFS patients with mild disease

Gastric tissue metabolites-mitochondrial metabolites correlation analysis revealed significant correlations between multiple microbial metabolites and multiple mitochondrial metabolites, suggesting that microbial metabolites could potentially influence mitochondrial metabolites (Figure 6a)