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After 01-Anisotropy Macro script has finished, navigate to 03-ROI Selection and press Run Using either the oval tool or the any shape tool draw regions of interest over the largest portion of where the sensor is appearing in the Para channel Each independent trial consists of at least five to more than 30 cells (530 technical replicates)

Abbreviations BMI: Body Mass Index DNA: Deoxyribonucleic Acid DUSP1: Dual Specificity Phosphatase 1 ESAM: Edematous Severe Acute Malnutrition F/B: Firmicutes to Bacteroidetes ratio FTO: Fat Mass and Obesity-Associated Gene GH: Growth Hormone GPCR: G-Protein Coupled Receptors GPx3: Glutathione Peroxidase 3 GRP78: Glucose-Regulated Protein 78 H3Ac: Histone H3 Acetylation H3K20me3: Histone H3 Lysine 20 Trimethylation H3K4me3: Histone H3 Lysine 4 Trimethylation H3K9me: Histone H3 Lysine 9 Mono- or Di-Methylation H3K9me3: Histone H3 Lysine 9 Trimethylation H4Ac: Histone H4 Acetylation HDAC: Histone Deacetylase IDH1: Isocitrate Dehydrogenase 1 IGF-1: Insulin-like Growth Factor 1 IL-10: Interleukin 10 IL-18: Interleukin 18 IL-6: Interleukin 6 KAZN: Kazrin gene KMT1A: Lysine Methyltransferase 1 A (also known as SUV39H1) LPIN1: Lipin 1 LXR: Liver X Receptor Alpha MUAC: Mid-Upper Arm Circumference MAFLD: Metabolic DysfunctionAssociated Fatty Liver Disease NESAM: Non-Edematous Severe Acute Malnutrition NF-B: Nuclear Factor kappa-light-chain-enhancer of activated B cells PDE3A: Phosphodiesterase 3 A PDGFR: Platelet-Derived Growth Factor Receptor PMN: Peripheral Mononuclear Cells PPARG: Peroxisome Proliferator-Activated Receptor Gamma RNA: Ribonucleic Acid SAM: Severe Acute Malnutrition SCFA: Short Chain Fatty Acids SIRT1: Sirtuin 1 Gene SLC17A9: Solute Carrier Family 17 Member 9 T3: Triiodothyronine T4: Thyroxine TDH: L-Threonine Dehydrogenase TGF-: Transforming Growth Factor Beta TNF: Tumor Necrosis Factor TSH: Thyroid-Stimulating Hormone WHZ: Weight-for-Height Z-score mTOR: Mechanistic Target of Rapamycin References World Health Organization

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