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The animals were anesthetized by inhalation route with Isofluorane (Isboa Vet) using a vaporizer and intranasal administration was performed with two instillations of 20 l of the bacterial suspension prepared in PBS
It's not just about adding water to a powder
For a sense of how research observations with this compound are typically framed over time, see the 5-Amino-1MQ results timeline
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