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sgRNAs were designed using CRISPick (Broad Institute), obtained from Integrated DNA Technologies (Coralville, IA), annealed, phosphorylated, Esp3I-digested, and ligated into the purified digested backbones using Quick Ligase (New England Biosciences)
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Excessive vitamin A intake, especially from high-dose supplements, has also been linked to lip lesions and other skin issues
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