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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)
Low supply may reflect diet, absorption, ATP limitations, increased demand or poor recycling
By incorporating L-cysteine in the form of N-acetyle cysteine (NAC) into fish diets, aquaculturists can enhance the ability of fish to combat oxidative stress, which is crucial for protecting cellular integrity and supporting immune function [80, 98]
Stem Cell Rev and Rep 18 , 585594 (2022)
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