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After final three 5-min washes in each of 0.1% PBS-T and PBS, the blots were imaged on Odyssey CLx (LICORbio) with Image Studio Lite software
KPV , finally, is the tripeptide Lys-Pro-Val, the C-terminal fragment (residues 11 to 13) of the alpha-melanocyte-stimulating hormone (alpha-MSH)
For deficiency without neurological involvement or for borderline levels, oral supplementation with high-dose cyanocobalamin (1000-2000 mcg daily) is often effective, as approximately 1% of an oral dose is absorbed through passive diffusion even in the absence of intrinsic factor
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