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To generate the GFP-fusion of Go-Nt 7 and Go-Nt 31 , the fragments were PCR-amplified (primers listed in Supplementary Data 3) from Go-GFP, products were cut with KpnI/AgeI and cloned in frame into the same sites of pEGFP-N1 (Clontech)
10.1074/jbc.M112.402776 79 MathieuY.ProsperP.FavierF.HarvengtL.DidierjeanC.JacquotJ
Other people at risk of deficiency include those who have had intestinal surgery, including weight loss surgery
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