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The mechanism of action involves binding to GLP-1 receptors found predominantly in pancreatic beta cells, where they enhance glucose-dependent insulin secretion
-arrestin 2 recruitment HEK293T cells (3.2 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 g plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and -arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent
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Total RNA was isolated with the TRIzol reagent and centrifuged at 12,000 g for 15 min at 25C following the addition of chloroform
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