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The results of our study indicate that the major components of FRP can take effect on multiple targets of this signaling pathway (e.g., AKT1, EGFR, IGF1R, et al.)
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Topical formulations were subsequently developed and approved for hair loss use: first for men (1988) and later for women (1991)
Briefly, once proteins have been spotted, the membrane strips were dried and then non-specific sites were blocked by soaking in 5% BSA in TBS-T (20 mM Tris-HCl, 150 mM NaCl, 0.05% Tween20) for 30 minutes
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