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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min
& Feldman, S
Bacteriostatic water supports controlled reconstitution of LPS-free peptides by reducing the risk of microbial proliferation during repeated vial access, helping preserve low endotoxin conditions critical for sensitive research assays
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