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After the recipient mice were fasted overnight for 16 h, the prepared liquid was administered orally and food intake was measured after 1, 3, 6, and 24 h
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The viral phenotype was confirmed by pyrosequencing the NA gene (WT and Tamiflu-resistant) (Fig
The process is relatively slow, and takes hours, and is also affected by diurnal variation, with less excretion overnight when urine flows tend to be low to allow sleep, and higher during the day [13]
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