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Many of the studies are conducted following a randomized, double-blind and placebo-controlled (RDP) methodology to assess the efficacy of the extract in comparison to potential placebo effects
For the invasion assay, Matrigel (BD, cat
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The positive effects of the diet are attributed to maintaining a state of nutritional ketosis, which is also a marker for compliance to the diet and assessed by elevated levels of ketone bodies such as acetoacetate in the urine, beta-hydroxybutyrate (BHB) in the blood, and acetone in the breath (7, 8)
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