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Library preparation and next-generation sequencing Lysates were homogenized and denatured at 95 C for 5 min, and equal lysate volumes from each treatment well were added to a custom PCR master mix for PRISM cell barcode amplification

Timing fiber intake, particularly soluble fiber, around meals can help to minimize postprandial glucose spikes by slowing down the absorption of glucose during digestion, leading to a more gradual rise in blood glucose and lasting feelings of satiety following meals

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GLP-1 agonists for type 2 diabetes: pharmacokinetic and toxicological considerations