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Using distilled water, tap water, or saline introduces contaminants or changes the solution chemistry in ways that can damage the peptide or cause adverse reactions
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In aged rat models, treatment increased activity of antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase, and glutathione-S-transferase, improving overall oxidative defense
That said, the gene expression data involving hundreds of neuronal genes suggests this could become a significant application area as human studies catch up
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