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Transepithelial resistance measurements Caco-2 cells were seeded onto 12 mm polycarbonate membrane inserts (PCF membrane inserts, Millipore, Germany) and bathed in DMEM/F12 medium which contained phenol red, 10% FBS, penicillin (100 units/ml) and streptomycin (10 g/ml)

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In contrast, Type II tumors, dominated by high-grade serous carcinoma, are highly aggressive, genetically unstable, and almost universally defined by mutations in TP53 [5]