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The advantage of on-column tag removal is that it eliminates the extra step of separating the cleaved protein from GST, since the GST-tag itself remains bound to the medium
Instead, it contains a compound called silymarin, which is designed to help the body increase its own internal production of glutathione and protect existing levels from being depleted
A phylogenetically tree showed that GpoA present a higher homology with genes found in bacteria that belong to the Phylum Bacillota (before known as Firmicutes ), mainly in the genus Streptococcus (Fig S1)
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