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Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h

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The worsening is due to the correlation between reduced melatonin levels and pathologies such as Alzheimers onset, menopause severity, and cardiovascular disorders.7 Melatonin comes from the pineal gland, epitalon is a pineal gland peptide, and clinical research points to a close relationship between the two