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In murine models, Spinacia oleracea -derived CQDs showed safe distribution at concentrations up to 100 g/mL with no histological organ damage, whereas higher doses elicited mild liver lymphocyte infiltration [ Furthermore, cobalt-conjugated N-CQDs demonstrated efficient penetration into neuronal and non-neuronal tissues in zebrafish, a well-established non-mammalian vertebrate model, without eliciting detectable apoptosis or oxidative stress [161, 163]

Notably, controlled fluid flow introduces sustained perfusion and physiologically relevant shear forces, both of which modulate cellular behaviour and allow for a more accurate assessment of nanoparticle transport, distribution and cellular uptake than static in vitro models [175, 176]

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