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Protein concentration of lysates was measured using Pierce BCA Protein Assay (Thermo Fisher Scientific)
Treatment of phLF and phBEC with TGF-1, TNF-, and menadione For treatment of phLF, cells were seeded at a density of approximately 20,000 cells/cm 2 , starved for 24 hours in DMEM/F12 with 0.5% FBS and 100 nM sodium selenite, followed by treatment with 2 ng/ml TGF-1 (R&D Systems, Minneapolis, MM, USA), 10 ng/ml TNF- (PeproTech, Rocky Hill, NJ, USA), and 10 M menadione (Sigma-Aldrich) in starvation medium for the indicated time points
MTHFR and Vaccinations: The MTHFR gene codes for the enzyme that plays a critical role in using folate in the methylation cycle
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