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To perform qPCR, 1 l of cDNA was mixed with 5 l of SyBR master mix (Roche), 1 l of forward primer (10 M) and 1 l reverse primer (10 M) on 96-well plates

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In summary, the developed IGF-1 MSIA-SRM proved effective and efficient in enabling the measurement of IGF-1

GW, FK, TG, and JM designed the experiments and wrote the manuscript