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GST pulldown assay, SPR assay, and intracellular cAMP accumulation assay identified three GLP-1RA candidates (D13, D41, D44) with strong receptor binding and effective functional activity, making them suitable for further in vivo experimental evaluation
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Given that liraglutide and semaglutide comprised the majority of studies, the results are most applicable to these agents, although the pooled analysis incorporated data from the entire GLP-1 RA class
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