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After being collected in 500 l of PBS the capillary fraction was divided into three aliquots, one for the maximum LDH release, one for the vehicle treatment induced LDH release (spontaneous LDH release) and one for the treatment induced LDH release
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The tissues were dewaxed and rehydrated through a series of xylene and ethanol changes
Step 1: Prepare Sterile Materials and Calculate Reconstitution Volume Before opening any vial, assemble all materials in a clean workspacepreferably a dedicated research area free from airborne contaminants
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