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For activity screening, we adapted a GSTO1 substrate assay that relies on the quantification of the reduction of S -(4-nitrophenacyl)glutathione (4-NPG), a GSTO1-specific substrate 17 , using recombinant human GSTO1 in a high-throughput screening format

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Hagen TM, Ingersoll RT, Wehr CM, Lykkesfeldt J, Vinarsky V, Bartholomew JC, Song MH, Ames BN: Acetyl-L-carnitine fed to old rats partially restores mitochondrial function and ambulatory activity

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