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The expression of ZO-1, Occludin, SIRT1, STAT3, p-STAT3, RORt, IL-6, IL-1, TNF-, and -actin was examined using the method previously described (Yao et al., 2014)
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For instance, a study by Grace et al., on lambs receiving a single 2 mg subcutaneous B12 injection demonstrated significant increases in serum and liver B12 concentrations for up to 24 days compared to untreated animals (2)
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