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The 10-day window is intended to deliver a concentrated signaling effect to the cells involved in circadian regulation and renewal before the long rest allows those adaptations to consolidate
The MDM is straightforward with minimal risk
For NanoLuc-only controls, empty pcDNA3.1(+) vector was used to maintain a constant amount of total transfected DNA
Cerebral folate deficiency with developmental delay, autism, and response to folinic acid
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