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After the injection, the swelled pancreata were isolated and incubated with 2 ml of pancreas lysis buffer in a 50-ml tube with a 37 C water bath for 15 min

& Aebersold, R

Dehydroascorbate reductase AsA, which is a major anti-oxidant in plants, is oxidized to DHA via successive reversible electron transfers with MDHA as a free radical intermediate