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At a separate -arrestin recruitment pathway at GLP-1R, reta E_max = 40% of native (partial / biased agonism), tirzepatide partial in the same direction, semaglutide full at both pathways
Fractions were collected using Piston Gradient Fractionator (Biocomp) and Econo UV monitor (Bio-Rad)
Old individuals are more susceptible to deficiency
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