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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

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Reported side effects tend to be mild and localized, including: Temporary redness or irritation at injection or application sites Mild itching Rare allergic sensitivity in individuals with copper sensitivities Who Should Avoid GHK-Cu

As the body ramps up red blood cell production in response to B12, potassium can be used up, so your provider may recommend monitoring it with a blood test