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An iScript cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to amplify 1 g of RNA per reaction in a MyCycler thermal cycler (Bio-Rad)
Since 90% of the bodys serotonin originates in the gut, these peripheral effects could influence central serotonergic tone through vagal signaling
Cushman CJ, Ibrahim AF, Smith AD, Hernandez EJ, MacKay B, Zumwalt M
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