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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample
Agrawal KK, Murti Y, Tangeretin
(PubMed) Fratantonio D, Speciale A, Molonia MS, et al
This resulted in a dramatic reduction of the high-energy barriers previously obtained with DFT or QM/MM methods, often to zero, enabling the study of vitamin B12 cofactors in in vivo processes
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