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Mice were deeply anaesthetized and perfused transcardially with 30 ml of PBS (pH 7.4) followed by 30 ml of cold 4 % paraformaldehyde (PFA) in PBS

Mean values per animal were calculated, between group comparisons made using the Kruskal-Wallis non-parametric test followed by Dunns post hoc test

This technique is particularly effective for confirming known interactions and mapping interaction sites, and is extensively used in basic research, drug screening, and exploring disease mechanisms

These peptides are often integrated with nutrient optimization, hormone support, or regenerative treatments for a comprehensive approach