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Immunostaining of GABA A R-1 subunits in the DCN Animals used for brainstem sectioning and subsequent immunohistological evaluations in each experimental group (68 rats/group) were evaluated for sufficient level of sedation after ABR and if necessary given additional ketamine at half the initial dose, then euthanized with i.p
A 30 mg vial used over five weeks at 6 mg per week experiences five punctures
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