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Afterward, the PBS, NP2, and NP4 solutions were bubbled with the flowing O 2 , where the O 2 concentration was monitored using a portable dissolved oxygen meter (Hanna S20 Instruments HI-2004-02 Edge Dissolved Oxygen Meter) for 5 min
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Consequently, deficiency of the neutrophil chemokine, Cxcl1, or antibody-mediated depletion of neutrophils reduces blood biomarkers of hepatocyte injury in APAP-treated mice ( APAP-induced ALF patients with the poorest prognosis develop compensatory anti-inflammatory response syndrome (CARS), a condition characterized by severe systemic immune suppression ( Liver injury after APAP overdose stimulates the hepatic release of Ccl2, a chemokine that facilitates the recruitment of Ly6C hi monocytes to the damaged liver in a Ccr2-dependent manner ( Materials and methods Animals and treatments 12-16-week-old male C57BL/6J or IL-10 reporter mice (B6.129S6-Il10 tm1/flv /J
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