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Briefly, after surface staining, cells were fixed in FA and permeabilized with Triton 0.1%
The removal of superoxide radicals by SOD prevents the formation of the much more damaging peroxynitrite ONOO (O 2 + NO ONOO ) and maintains the physiologically relevant level of nitric oxide (NO ), an important molecule in neurotransmission, inflammation, and vasodilation
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