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It simply reflects fewer B12 molecules per milliliter of solution
Freezing at -20C extends this further
Funding This work was supported by National Key R&D Program of China (2019YFA0906100 and 2021YFC3300101), National Natural Science Foundation of China (82071772), Guangdong Basic and Applied Basic Research Foundation (2022A1515010070), Shenzhen Basic Science Research Project (JCYJ20220818102018038, JCYJ20220531095612029, JSGGZD20220822100005011), Shenzhen Medical Research Fund (A2301047), and Futian Healthcare Research Project (FTWS2023066)
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