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Mechanistically, PA reduces the expression of GPX4 and heat shock factor 1 and regulates the transcription of genes related to iron metabolism (such as FTH, TFR1, and SLC40A1), causing iron homeostasis imbalance, while GPX4 overexpression reverses PA-induced ferroptosis in cardiomyocytes
The results of the HLC differentiation correlate to the initial results of the EP screen in two critical aspects: first, only the lines which passed the EP screen are capable of differentiation into HLCs
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