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The animal behavior analysis software should be initiated to automatically record the animals activity within the box, typically for a duration of 15 min

Pre-cycle testing Before starting IGF-1 LR3, establish baseline values for: Fasting glucose and insulin HbA1c (long-term blood sugar marker) IGF-1 levels Complete metabolic panel Lipid panel These baselines allow comparison during and after cycles to identify any negative trends

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ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability