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a 2006 clinical trial found CJC-1295 alone produced 1.5- to 3-fold IGF-1 increases lasting 9-11 days after a single dose Administration: Subcutaneous injection Typical dosing range: 100-300 mcg of each peptide per injection, once daily (typically before sleep on an empty stomach) Cycle length: 12-24 weeks

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Quality control Four quality control (QC) samples were prepared by pooling 20 L of each sub-aliquoting corresponding sample into a new tube, and MCF derivatization was prepared in the aforementioned manner

Samples were run in duplicate following the manufacturers protocols, with the exception of longer sample and calibrator incubation for cytokine plates (overnight instead of 2 hours at 4C)