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In the Eqn.4 L is the concentration of the competing ligand, K 1 and K 2 correspond to the dissociation constants of CuL and CuL 2 complexes, respectively [Cu] total is the concentration of copper and [Cu] bound to protein is the concentration of the copper bound to the protein at IC50
These are generally mild and often resolve with time or dose adjustment
For twice-daily protocols, aim for a 10-12 hour separation (e.g., 8 AM and 8 PM)
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