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For the targeted sulfur metabolite quantification approach, the previously established LC-MS conditions were applied 99 with selected reaction monitoring (MRM) using an Ultimate 3000 UPLC system coupled to a Thermo Finnigan TSQ Quantum equipped with HESI (Thermo Fisher Scientific, Waltham, MA)
Follow-up Repeat broad markers after 6-12 weeks if the protocol continues, symptoms change, or there are concerns about inflammation, infection, bruising, swelling, or recovery quality
Over the following weeks, patients often report smoother movement, improved tissue quality, and faster recovery from disc irritation, joint inflammation, or tendon strain
Blood 2005
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