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Whether a given study design benefits from synchronising with that pulse, or deliberately offsetting it, depends on the readout being measured, because the timing affects whether the exogenous GH signal amplifies or dampens the endogenous pulse

Complete the testing while not using any compounds that might affect results

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It is noteworthy that hybrids GG2[D u A u A u ] 9,10,11,12 ( 2 , 13 nM) and GG2[F u I u A u ] 22,23,24,25 ( 16 , 62 nM) which contain native side chains and shifted substitution pattern still preserve substantial affinity with GLP-1R, although with over a hundred fold loss compared to the native peptide