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Histochemical GUS staining Seedlings of the cell cycle reporters were submitted to short- or long-term treatments, after which whole seedlings were immersed in GUS assay buffer with minor modifications depending on the line used

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Why it helps: Protects from light Adds insulation against temperature fluctuations Keeps vials organized with reconstitution dates Label with reconstitution date Essential practice: Write reconstitution date on vial or box immediately after mixing