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In the diabetes trial, the primary endpoint was a change in glycated hemoglobin (HbA1c) values from baseline to week 24

Vitamin B12 is one of the 8 B vitamins that is an essential nutrient for proper bodily functions

Painful stones, inflammation, or a gallbladder that empties poorly (confirmed by HIDA scan) are the usual reasons to remove it

The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)