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Membranes were collected by centrifugation (30,000 rpm) at 4 C for 30 min, and solubilized in 0.5% (w/v) LMNG, 0.1% (w/v) CHS, 10 M peptide 20, 2 mM MgCl 2 , 100 U salt active nuclease and 25 mU/mL apyrase for 2.5 h at 4 C