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The 1st glutathione-interaction site, also termed glutathione-scaffold site, is rather well defined and comprises several protein areas that altogether contribute to catalysis by interacting with the glutathione moiety of GSSR 7,8,13,14,15,35 , therefore explaining the specificity for the -glutamyl moiety 9,49,50

Yeast strains carrying a conditional mutation in Ero1 were compromised in their ability to form disulphide bonds in secreted proteins when grown at the nonpermissive temperature

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