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[91] King's laboratory was given some hexuronic acid by Szent-Gyrgyi and soon established that it was the sought-after anti-scorbutic agent
Structural studies describe coordination through the imidazole nitrogen of the histidine residue together with the terminal amine and an adjacent amide nitrogen, forming a stable square-planar copper complex
Apply gentle pressure with a clean cotton ball or gauze
After washing with PBS, the coverslips were incubated with 0.5% Triton X-100 for 10 min
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