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They were acclimatized in well aerated cages with alternating 12 hour light and dark cycles, maintained on standard rat chow and allowed free access to drinking water according to the regulations and ethics governing the use of animals in laboratory experiments of the University of Ibadan
Appetite suppression is a secondary effect but not a primary purpose
Importantly, the fusion proteins that remained bound to the immobilized receptor at the end of the dissociation phase, rapidly dissociated upon injection of a pH 7.4 buffer during the regeneration phase (Supplementary Fig
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