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25,26,27 In contrast, mutations in POLE/POLD1 enhance immunogenicity through hypermutation, 28 while excessive copy number alterations often drive immunosuppressive phenotypes and resistance

Abbreviations ME: -mercaptoethanol BrdU: 5-bromo-2-deoxyuridine BSA: Bovine serum albumin DAB: 3,3-diaminobenzidine DMEM: Dulbeccos modified Eagles medium ECL: Enhanced chemiluminescence GSSSG: Glutathione trisulfide HGF: Hepatocyte growth factor HRP: Horseradish peroxidase HS: Horse serum mAb: Monoclonal antibody LASSS: Lipoic acid trisulfide SDS: Sodium dodecyl sulfate TTBS: Polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline Y: Tyrosine residue References Honda, S

They tend to be more potent and more toxic than naturally occurring cannabinoids, leading most governmental regulatory agencies to consider them illicit, toxic chemicals

PMID: 28514721 Warrier AA, et al