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Also, individuals who have undergone certain surgical procedures, including intestinal resection, bypass, or bariatric surgery, which may reduce Vitamin B12 absorption, are also eligible

Conclusion Proper storage of lyophilised IGF-1 LR3 at 2-8C (or -20C for extended storage), careful reconstitution using bacteriostatic water with proper aseptic technique, and responsible handling of reconstituted solutions are critical for maintaining research integrity

For Western blotting and immunofluorescence staining, the following antibodies were used: LOXL2 rabbit monoclonal (Abcam, ab179810), LOX rabbit polyclonal (Invitrogen, PA1-46020), LOXL1 mouse monoclonal (Santa Cruz, sc-166632), LOXL3 rabbit polyclonal (Santa Cruz, sc-68939), VAP-1 (AOC3) mouse monoclonal antibody (Santa Cruz, sc-373924), Collagen I monoclonal antibody (Invitrogen, MA1-26771), Collagen IV polyclonal antibody (Assay Biotech, C0157), GAPDH mouse monoclonal (Novus Bio, NB300221), goat anti-mouse IgG (H + L)-HRP conjugate (Biorad 1706516), AffiniPure goat anti-rabbit IgG (H + L)-HRP conjugate (Jackson ImmunoResearch, 111035144), Cy5 AffiniPure Goat Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch, 111175144), Alexa Fluor 568 Goat anti-Rabbit IgG (H + L) Secondary Antibody (Invitrogen, A-11011), Alexa Fluor 488 Donkey anti-Mouse IgG (H + L) Secondary Antibody (Invitrogen, A-21202) and fluorescein (DTAF) streptavidin (Jackson ImmunoResearch, 016010084)